WP7 - The pathogenicity of RNA polymerase and NS1 suppression on IFN-α/β production in different strains of avian flu viruses (AIV) from China

Partners involved:
N° 8, Prof. Bing Sun (WP Leader)
N° 5, Dr. Ervin Fodor
N° 6, Dr. Nadia Naffakh

Objectives
The aim of WP7 is to compare the function of RNA polymerase on pathogenesis and NS1 suppression on IFN-α/β production in different strains of avian flu viruses (AIV) from China. The sequence comparison and domain swapping experiment will be performed in RdRp and NP, and their structural and biochemical differences will be confirmed in replicon and RdRp activity in vitro. Sequence analysis of PB2, NP and NS1 from highly pathogenic avian influenza viruses (HPAIV) H5N1 strain and fatal human infection cases in China will be performed and the potential functional regions will be predicted. Multiple mutation will be performed in PB2 and NP molecules and their function and biochemical differences RdRp will be confirmed in vitro. Since IFN-α/β production is critical for virus replication in host cells, avian flu virus-triggering TLR signal pathway and new molecules from host cells that regulate IFN- production will be investigated. Finally, inflammatory cytokine and chemokine production in epithelial cells of lung in response to virus infection will also be investigated.

WPs